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KMID : 1234420100380010070
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 1 p.70 ~ p.76
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Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes
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Bae Yun-Ui
Moon Ik-Jae
Seu Young-Bae
Doh Kyung-Oh
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Abstract
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The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13 cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarray and antisense libraries for an effective functional validation of genes.
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KEYWORD
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Microarray, expression profiling, microarray, antisense, lung cancer
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